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Stormont's department of finance has responsibility for marriage regulations in Northern Ireland.
In response to the publication, a DUP spokesman said: "This correspondence was exploring technical legal matters and was not personally driven by the minister.
The Scottish Government has published a written request from DUP leader Arlene Foster asking that it restrict gay couples from Northern Ireland converting their civil partnerships to same sex marriages in Scotland.
The publication comes days after Mrs Foster said she had no recollection of sending such correspondence to the administration in Edinburgh.
"If I'd written to him officially as minister of finance or something like that around recognition laws here in Northern Ireland, I have no recollection of it.
I certainly didn't write in a personal capacity." The letter dated September 4 2015 released by the Scottish Government on Tuesday is signed by Mrs Foster.
Mrs Foster wrote: "I'm sure neither of us would wish to place same sex couple in an uncertain legal position, which maybe difficult and expensive to resolve." Mr Biagi rejected the request from the Northern Ireland ministers.
"I'm not quite sure what he (Mr Biagi) was referring to but it certainly wasn't a letter from me and I've no recollection of a letter from me," she told BBC Radio Ulster's Inside Politics show.
The letter, written in September 2015 when Mrs Foster was Stormont finance minister, urged then Scottish local government minister Marco Biagi to exclude Northern Ireland-based couples from legislation that enabled people in civil partnerships to convert those unions to same sex marriages.
Read more: Independence referendum plans statement 'likely' before Holyrood recess Mr Biagi tweeted about the existence of the letter in the wake of the General Election, amid increased UK-wide focus on the DUP's conservative stance on social issues such as gay marriage.
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